Quantitative in situ proximity ligation assays examining protein interactions and phosphorylation during smooth muscle contractions
نویسندگان
چکیده
منابع مشابه
Improvements and Applications of in situ Proximity Ligation Assays
Grannas, K. 2015. Improvements and Applications of in situ Proximity Ligation Assays. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1099. 48 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-554-9233-5. The cells building up the human body is in constant communication with each other. This communication is done through large complex networks of sig...
متن کاملQuantifying RNA–protein interactions in situ using modified-MTRIPs and proximity ligation
The stabilization, translation and degradation of RNA are regulated by interactions between trans-acting factors, such as microRNA and RNA-binding proteins (RBP). In order to investigate the relationships between these events and their significance, a method that detects the localization of these interactions within a single cell, as well as their variability across a cell population, is needed...
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Löf, L. 2016. Applications of in situ proximity ligation assays for cancer research and diagnostics. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1241. 56 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-554-9638-8. In the field of cancer research and diagnostics it is crucial to have reliable methods for detecting molecules involved in the disea...
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Protein-binding DNA sequence elements encode a variety of regulated functions of genomes. Information about such elements is currently in a state of rapid growth, but improved methods are required to characterize the sequence specificity of DNA-binding proteins. We have established an in vitro method for specific and sensitive solution-phase analysis of interactions between proteins and nucleic...
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Many studies have demonstrated an association between the cytoskeleton and mRNA, as well as the asymmetric distribution of mRNA granules within the cell in response to various signaling events. It is likely that the extensive cytoskeletal network directs mRNA transport and localization, with different cytoskeletal elements having their own specific roles. In order to understand the spatiotempor...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2019
ISSN: 0003-2697
DOI: 10.1016/j.ab.2019.04.009